Kinetics of polymer looping with macromolecular crowding: effects of volume fraction and crowder size
نویسندگان
چکیده
منابع مشابه
Kinetics of polymer looping with macromolecular crowding: effects of volume fraction and crowder size.
The looping of polymers such as DNA is a fundamental process in the molecular biology of living cells, whose interior is characterised by a high degree of molecular crowding. We here investigate in detail the looping dynamics of flexible polymer chains in the presence of different degrees of crowding. From the analysis of the looping-unlooping rates and the looping probabilities of the chain en...
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DNA-binding proteins control how genomes function. The theory of facilitated diffusion1 explains how DNA-binding proteins can find targets apparently faster than the diffusion limit by using reduced dimensionality2,3—combining threedimensional (3D) diffusion through cytoplasm with 1D sliding along DNA (refs 3–15). However, it does not include a description of macromolecular crowding on DNA as o...
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We have examined the effect of crowder particle charge on macromolecular structure, studied via small-angle neutron scattering, and translational dynamics, studied via pulsed-field gradient NMR, in addition to bulk viscosity measurements, in a polymer macromolecule (polyethylene glycol)-nanoparticle crowder (polysucrose, Ficoll70) model system, in the case where polymer size and crowder size ar...
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ژورنال
عنوان ژورنال: Soft Matter
سال: 2015
ISSN: 1744-683X,1744-6848
DOI: 10.1039/c4sm02007c